Martin Wilding
DATE AND PLACE OF BIRTH: 3rd. April, 1969, London, England
NATIONALITY: British
WORK ADDRESS: CFA, Clinica Villa del Sole, 80123 Naples, ITALY.
CURRENT POSITION: Senior Embryologist.
UNIVERSITY EDUCATION:
Degree: 1987-1990 Imperial College, London, UK. B.Sc. (Hons) Biochemistry. Awarded July 1990
Post-grad.: 1991-1994 University College, London, UK. Ph.D. (Tutor: Prof. Michael Whitaker) Awarded July 1995
PROFESSIONAL CAREER
1990 Associate of the Royal College of Science, UK
1995-present Senior Embryologist, CFA, Clinica Villa del Sole, Naples, ITALY
1995-1997 Research Fellow (EEC Human Capital and Mobility Grant), Stazione Zoologica, Naples, ITALY
1997-1999 Internal Research Fellow (Art. 23) Stazione Zoologica, Naples, ITALY
1998 Member of the Institute of Biology, UK.
1999-2002 Clinical Associate, Laboratory of In Vitro Fertilisation, University of Naples ‘Federico II’, ITALY
2000-2001 External collaborator, Laboratory of In Vitro Fertilisation, Istituto Materno Infantile, Palermo, ITALY
2001-2006 Member of the ‘European Countries Biologists Association’
2002 Granted licence as ICSI practitioner, HFEA, UK.
2004 Registered with Italian Order of Biologists
2005-2006 Teramo, Italy. Lectures in Degree course, University of Teramo
2005-2008 Caserta, Italy. Lectures in Degree course, II University of Naples
2006 External Collaborator, Laboratory of In Vitro Fertilisation, University of Naples ‘Federico II’, ITALY
CONFERENCES AND COURSES ATTENDED
03/1995 Bourn Hall Clinic, Cambridge, UK. Participant in workshop ‘Intracytoplasmic sperm injection’.
06/1995 Porto Cervo, Sardinia, ITALY. Speaker, international meeting on infertility.
05/1996 Clinica Villa del Sole, Naples, ITALY. Demonstrator in workshop ‘Micromanipulation in Embryology’
05/1997 Vietri sul Mare, ITALY. Speaker in Serono Symposium ‘Stato del Arte sulla Fecondazione Assistita’
06/1997 Università degli studi di Bari. Speaker in workshop ‘Micromanipolazione dei Gameti’
11/1997 Sorrento, ITALY. Speaker in Serono symposium ‘Development of the human embryo in vitro’
10/1998 Clinica Villa del Sole, Naples, ITALY. Demonstrator in Alpha workshop ‘Micromanipulation in Embryology’
05/1999 Clinica Villa Igea, Foggia, ITALY. Speaker in congress ‘La sterilità di coppia’
04/1999 Clinica Villa del Sole, Naples, ITALY. Demonstrator in Alpha international workshop ‘Micromanipulation in Embryology
09/1999 Modena, ITALY. Speaker in international meeting on fertility
10/1999 Clinica Villa del Sole, Naples, ITALY. Speaker ’New techniques in prenatal diagnosis’
11/1999 Clinica Villa del Sole, Naples, ITALY. Demonstrator in Alpha workshop ‘Micromanipulation in Embryology’
05/2000 Naples, ITALY. Speaker, SIGO conference.
01/2001 Bombay, INDIA. Demonstrator in ‘II Indo-Italian In Vitro Fertilisation Workshop’.
09/2001 New York, USA. Speaker, Alpha 2001 congress.
10/2001 Campobasso, ITALY. Speaker, Serono symposium.
12/2001 Naples, ITALY. Speaker, Italian Society of Reproduction
07/2002 Vienna, Austria. Speaker, ESHRE annual meeting
03/2008 Gröningen, Holland. Speaker, 12th Reiner de Graaf Symposium
07/2011 Stockholm, Sweden.Invited Speaker, EHSRE conference
PUBLICATION RECORD:
FULL PAPERS
Ciapa, B., Pesando, D., Wilding, M. and Whitaker, M. (1994).Cell cycle calcium transients driven by cyclic changes in inositol trisphosphate levels. Nature (Lond.)368, 875-878.
Wilding, M., Török, K. and Whitaker, M. (1995).Activation-dependent and activation-independent localisation of calmodulin to the mitotic apparatus during the first cell cycle of the Lytechinus pictus embryo. Zygote 3, 219-224.
Wilding, M., Wright, E., Patel, R., Ellis-Davis, G. and Whitaker, M. (1996). Local perinuclear calcium signals associated with mitosis-entry in early sea urchin embryos. J. Cell Biol. 135, 191-199.
Wilding, M. (1996).Calcium and cell cycle control in early embryos. Zygote 4, 1-6.
Wilding, M., Kyozuka, K., Russo, G.L., Tosti, E. and Dale, B. (1997). A soluble extract from human spermatozoa activates ascidian eggs. Dev, Growth Diff., 39, 329-336.
Wilding, M. and Dale, B. (1997). Sperm Factor, what is it and what does it do?. Mol. Hum. Reprod., 3, 269-273.
Grumetto, L., Wilding, M., De Simone, M., Tosti, E. Galione, A. and Dale, B. (1997). Nitric oxide triggers the fertilisation current in ascidians. Biochem. Biophys. Res. Comm. 239, 723-728.
Dale, B., Menezo, Y., Cohen, J., Di Matteo, L. and Wilding, M. (1998). Intracellular pH regulation in the human oocyte. Hum. Reprod.13, 964-970.
Török, K., Wilding, M., Groigno, L., Patel, R. and Whitaker, M. (1998). Imaging the spatial dynamics of calmodulin activation during mitosis. Curr. Biol.8, 692-699.
Wilding, M., Menezo, Y., Cohen, J. and Dale, B. (1998). Regulation of intracellular pH in mammalian preimplantation embryos. Ass. Reprod. Rev 8, 36-39.
De Simone, M.L., Grumetto, L., Tosti, E., Wilding, M. and Dale, B. (1998). Non-specific currents at fertilisation in sea urchin eggs. Zygote 6, 11-15.
Wilding, M., and Dale, B. (1998). Soluble extracts from ascidian spermatozoa trigger intracellular calcium release independently of the activation of the ADP ribose channel. Zygote 6, 149-154.
Wilding, M., Russo, G.L., Marino, M., Dale, B., and Galione, A. (1998). ADP ribose gates the fertilisation current in ascidian oocytes. Am. J. Physiol. 275, C1277-C1283.
Wilding, M. (1998). FISH leads patients to successful treatment in Naples. Alpha magazine 13, 4-5.
Russo, G.L., Wilding, M., Marino, M. and Dale, B.(1998). Ins and outs of meiosis in ascidians. Sem. Cell Dev. Biol. 9, 559- 567.
Dale, B. and Wilding, M. (1999). Activating eggs…what triggers the action? Alpha magazine 16, 8-9.
Dale, B., Marino, M. and Wilding, M. (1999). Sperm-induced calcium oscillations. Mol. Hum. Reprod 5, 1-4.
Dale, B., Fiorentino, A., De Stefano, R., Di Matteo, L., Wilding, M. and Zullo, F. (1999). Activated oocyte transfer (AOT) as a stress-free approach to the treatment of infertility. Hum. Reprod. 14, 1771-1772.
Wilding, M., Marino, M., and Dale, D. (1999). Nicotinamide nucleotides trigger the destruction of MPF activity after fertilisation in ascidians. Zygote 7, 255-260.
Dale, B., Marino, M., and Wilding, M. (1999). The ins and outs of meiosis. J Exp. Zool. 285, 226-236.
Marino, M., Wilding, M. and Dale, B. (2000). Interaction of cell cycle kinases, microtubules and chromatin in ascidian oocytes during meiosis. Mol. Reprod. Dev. 56, 155-162.
Wilding, M., Marino, M. and Dale, B. (2001). The male centrosome defines the origin of post-fertilisation calcium waves during meiosis in ascidian oocytes. Zygote 8, 285-293.
Wilding, M., Dale, B., Marino, M., Di Matteo, L., Alviggi, C., Pisaturo, M.L., Lombardi, L. and De Placido, G. (2001). Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos. Hum. Reprod.16, 909-917.
Dale, B., Wilding, M., Botta, G., Rasile, M., Marino, M., Di Matteo, L., De Placido, G. and Izzo, A. (2001) CASE REPORT: Pregnancy after cytoplasmic transfer to reduce embryo fragmentation in a couple suffering from idiopathic infertility. Hum. Reprod. 16, 1469-1472.
De Placido, G., Alviggi, C., Carravetta, C., Pisaturo, M.L., Sanna, V., Wilding, M., Lord, G.M., Matarese, G. (2001). The peritoneal fluid concentration of leptin is increased in women with peritoneal but not ovarian endometriosis. Hum.
Reprod. 16, 1251-1254.
Mollo, A., Alviggi, C., Strina, I., Varricchio, M.T., Pisaturo, M.L., Carravetta, C., Alviggi, E., Lombardi, L., Schiattarella, C., Wilding, M., De Placido, G. (2001). Efficacy of human menopausal gonadotrophin for controlled ovarian hyperstimulation in poor responders to recombinant follicle stimulating hormone. In ‘ESGOI 2001’ (Madonna di Campiglio, Italy March 11-18, 2001). CIC Edizioni Internazionali, Roma, Italy. p 335-341.
Wilding M., Carotenuto, R., Infante, V., Dale, B., Marino, M., Di Matteo, L., Campanella, C. (2001). Confocal microscopy analysis of the activity of mitochondria contained within the ‘mitochondrial cloud’ during oogenesis in Xenopus laevis. Zygote 9, 347-352.
Infante, V., Amirante, R., Vaccaio, M.C., Wilding, M., Campanella, C. (2001). Enzyme activity in anuran spermatozoa
upon induction of the acrosome reaction. Zygote 9, 293-298.
De Placido, G., Mollo, A., Alviggi, C., Strina, I., Varricchio, M.T., Ranieri, A., Colacurci, N., Tolino, A., Wilding, M. (2001).Rescue of IVF cycles by HMG in pituitary down-regulated normogonadotrophic young women characterized by a poor initial response to recombinant FSH. Hum Reprod. 16,1875-1879.
De Placido, G., Wilding, M., Strina, I., Mollo, A., Alviggi, E., Tolino, A., Colacurci, N., De Matteo, L., Marino, M., Dale, B. (2002). The effect of ease of transfer and type of catheter used on pregnancy and implantation rates in an IVF program. J.Ass. Reprod. Gen 19, 14-18.
Wilding, M., Fiorentino, A., De Simone, M.L., Infante, V., De Matteo, L., Marino, M., and Dale, B. (2002). Energy substrates, the mitochondrial membrane potential and human preimplantation embryo division. Repro. Biomed Online 5, 39-42.
Dale, B., Wilding, M., Di Matteo, L., and Zullo, F. (2002). CASE REPORT: Absence of sperm sex chromosome aneuploidies in an XO/XYY male. Fertil. Steril. 78, 634-636.
De Placido G, Wilding M, Strina I, Alviggi E, Alviggi C, Mollo A, Varicchio MT, Tolino A, Schiattarella C, Dale B. (2002). High outcome predictability after IVF using a combined score for zygote and embryo morphology and growth rate. Hum.
Reprod. 17, 2402-2409.
Dale B., Fiorentino A., de Simone M., di Matteo L., Scotto di Frega A., Wilding M., Fehr P., Bassan E., Lo Giudice C., Maselli A., Cappiello F., Zullo F.(2002) Zygote versus embryo transfer: a prospective randomised multicentre trial. J. Ass. Repro. Gen. 19, 456-461.
Wilding, M., Fiorentino,A., De Simone, ML., Infante, V.,De Matteo, L. and Dale, B. (2002). Energy Substrates, mitochondrial membrane potential and human preimplantation embryo division. Reprod.Biomed. Online 5, 39-42.
Wilding, M., De Placido, G., De Matteo, L.Marino, M., Alviggi C., Dale, B. (2003). Chaotic mosaicism in human preimplantation embryos is correlated with a low mitochondrial membrane potential. Fertil. Steril. 79, 340-346.
Wilding, M., Forman, R., Hogewind, G., Di Matteo, L., Zullo, F., Cappiello, F., Dale, B. (2004). Preimplantation genetic diagnosis for the treatment of failed IVF-ET and habitual abortion. Fertil. Steril. 81, 1302-1307.
De Placido, G., Alviggi, C., Mollo, A., Strina, I., Ranieri, A., Alviggi, E., Wilding, M., Varricchio, M.T., Borelli, A.L., Conforti, S. (2004). Effects of recombinant LH (rLH) supplementation during controlled ovarian hyperstimulation (COH) in normogonadotrophic women with an initial inadequate response to recombinant FSH (rFSH) after pituitary downregulation. Clin. Endocrinol. 60, 637-643.
Scotto Di Frega, A., Dale, B., Di Matteo, L., Wilding, M. (2005). Secondary male factor infertility after Roux-en-Y gastric bypass for morbid obesity: case report. Hum Reprod. 20, 997-998.
Carotenuto, R., De Marco, N., Biffo, S., Wilding, M., Vaccaro, M.C., Marchisio, P.C., Capriglione, T., Russo,G.L., Campanella, C. (2005). Phosphorylation ofp27(BBP)/eIF6 and its association with the cytoskeleton are developmentally regulated in Xenopus oogenesis. Cell Mol Life Sci. 62, 641-1652.
Wilding, M., Di Matteo, L., Dale, B. (2005). The maternal age effect: a hypothesis based on oxidative phosphorylation. Zygote. 13, 317-323.
Wilding, M., Singer, M.,Fehr, P., Haeberlin, F., Roth, F., Lachat, R., Di Matteo, L., Capobianco, C., Dale, B. (2006). The effectof extended culture ofcumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles. J. Ass. Reprod. Gen 23, ì129-136.
De Placido, G., Alviggi, C., Clarizia, R., Mollo, A., Alviggi, E., Strina, I., Fiore, E., Wilding, M., Pagano, T., Matarese, G. (2006). Intra-follicular leptin concentration as a predictive factor for in vitro oocyte fertilization in assisted reproductive techniques. J Endocrinol Invest. 29, 719-726.
Wilding, M., Di Matteo, L., D’Andretti, S., Montanaro, N., Capobianco, C., Dale, B. (2007). An oocyte score for use in assisted reproduction. J Assist Reprod Genet. 24, 350-8.
Carotenuto R, Petrucci TC, Correas I, Vaccaro MC, De Marco N, Dale B, Wilding M. (2009). Protein 4.1 and its interaction with other cytoskeletal proteins in Xenopus laevis oogenesis.Eur J Cell Biol.88, 343-56.
Wilding M., Coppola G, Dale B, Di Matteo L.(2009). Mitochondria and human preimplantation embryo development. Reproduction 137, 619-24.
Dale B, Wilding M, Coppola G, Tosti E. (2010) How do spermatozoa activate oocytes? Reprod Biomed Online. Feb 14.
Wilding M., Coppola G, Dale B, Di Matteo L. (2009).Mitochondria and human preimplantation embryo development. Reproduction. 137:619-24.
Wilding MG, Capobianco C, Montanaro N, Kabili G, Di Matteo L, Fusco E, Dale B. (2010). Human cleavage-stage embryo vitrification is comparable to slow-rate cryopreservation in cycles of assisted reproduction. J Assist Reprod Genet. 27, 549-54.
Wilding, M., Scotto di Frega, A., Kabili, G., Di Matteo, L., Dale, B. (2010). Oocyte degeneration after intracytoplasmic sperm injection. J. Reprod. Stem Cell Biology 1; 196-198.
Wilding M., Coppola G, di Matteo L, Palagiano A, Fusco E, Dale B. (2011). Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J Assist Reprod Genet. 28, 253-62.
Vaccaro MC,Wilding M, Dale B, Campanella C, Carotenuto R. (2011). Expression of XNOA 36 in the mitochondrial cloud of Xenopus laevis oocytes. Zygote 15, 1-6.
BOOK CHAPTERS
Wilding, M., Russo, G.L., Marino, M., Grumetto, L., De Simone, M.L., Tosti, E. and Dale, B.(1998). Activation of Ciona
intestinalis at fertilisation is controlled by nicotinamide nucleotide metabolism. In ‘New Developments in Marine Biotechnology’ (eds. Le Gal and Halvorson). Plenum Press, New York
Russo, G.L., Kyozuka, K., Marino, M., Tosti, E., Wilding, M., De Simone, M.L. and Dale, B. (1998). Meiotic cell cycle control by mos in ascidian oocytes. In ‘NewDevelopments in Marine Biotechnology’ (eds. Le Gal and Halvorson).Plenum
Press, New York. pp115-119.
Dale, B., Di Matteo, L., Marino, M., Russo, G. and Wilding, M. (1999). Soluble sperm activating factors. In ‘The male gamete’ (ed. C.Gagnon). Cache River Press, IL, USA. pp291-302.
Wilding M., Dale B., Pisaturo, M.L., Lombardi, L., Strina, I., Alviggi, E., Carravetta, C., De Placido, G.(2000). Analisi con microscopio confocale dell’attività mitocondriale durante le fasi di svillupo pre-impianto di embrioni umani. In ‘Atti Soc. It. Gyn.Ost.’ LXXVI Frat. Ferrari Editore, Naples, Italy. pp731-735
Wilding, M., Ranieri A., Strina, I., Alviggi, E., Alviggi, C., Mollo, A., Varicchio, M.T., De Placido G. (2000). Comparazione tra la qualità degli zigoti e degli embrioni e valore predittivo sull’outcome dei cicli di fecondazione in vitro. In ‘Atti Soc. It. Gyn.Ost.’ LXXVI Frat. Ferrari Editore, Naples, Italy. pp736-739.
Alviggi, C., Carravetta, C., Matarese, G., Pisaturo, M.L., Sanna, V., Wilding, M., Granata, P., De Placido, G. (2000). Livelli sierici ed intraperitoneali di leptina in pazienti con endometriosi. In ‘AttiSoc. It. Gyn. Ost.’ LXXVI Frat. Ferrari Editore, Naples, Italy. pp189-193.
Dale, B., Di Matteo, L., Wilding, M. (2007).Mitochondria in Reproduction: future assays for embryo selection. In ‘Human PreimplantationEmbryo Selection’ (eds K. Elder and J. Cohen) Informa healthcare. Pp275-286.
Contact Dr Martin Wilding here.
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